Improved internal control for molecular diagnosis assays

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Improved internal control for molecular diagnosis assays

The two principal determining steps in molecular diagnosis are the amplification and the identification steps. Accuracy of DNA amplification is primarily determined by the annealing sequence of the PCR primer to the analyte DNA. Accuracy for identification is determined either by the annealing region of a labelled probe for the real time PCR analysis, or the annealing of a sequencing primer for...

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ژورنال

عنوان ژورنال: MethodsX

سال: 2015

ISSN: 2215-0161

DOI: 10.1016/j.mex.2015.03.002